Synthetic biology tools for engineering Yarrowia lipolytica
نویسندگان
چکیده
منابع مشابه
Engineering Yarrowia lipolytica for Campesterol Overproduction
Campesterol is an important precursor for many sterol drugs, e.g. progesterone and hydrocortisone. In order to produce campesterol in Yarrowia lipolytica, C-22 desaturase encoding gene ERG5 was disrupted and the heterologous 7-dehydrocholesterol reductase (DHCR7) encoding gene was constitutively expressed. The codon-optimized DHCR7 from Rallus norvegicus, Oryza saliva and Xenapus laevis were ex...
متن کاملNew inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica
BACKGROUND The oleaginous yeast Yarrowia lipolytica is increasingly used as alternative cell factory for the production of recombinant proteins. At present, several promoters with different strengths have been developed based either on the constitutive pTEF promoter or on oleic acid inducible promoters such as pPOX2 and pLIP2. Although these promoters are highly efficient, there is still a lack...
متن کاملEngineering of Yarrowia lipolytica for production of astaxanthin
Astaxanthin is a red-colored carotenoid, used as food and feed additive. Astaxanthin is mainly produced by chemical synthesis, however, the process is expensive and synthetic astaxanthin is not approved for human consumption. In this study, we engineered the oleaginous yeast Yarrowia lipolytica for de novo production of astaxanthin by fermentation. First, we screened 12 different Y. lipolytica ...
متن کاملMetabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
BACKGROUND Limonene, a monocyclic monoterpene, is known for its using as an important precursor of many flavoring, pharmaceutical, and biodiesel products. Currently, d-limonene has been produced via fractionation from essential oils or as a byproduct of orange juice production, however, considering the increasing need for limonene and a certain amount of pesticides may exist in the limonene obt...
متن کاملEngineering of a high lipid producing Yarrowia lipolytica strain.
BACKGROUND Microbial lipids are produced by many oleaginous organisms including the well-characterized yeast Yarrowia lipolytica, which can be engineered for increased lipid yield by up-regulation of the lipid biosynthetic pathway and down-regulation or deletion of competing pathways. RESULTS We describe a strain engineering strategy centered on diacylglycerol acyltransferase (DGA) gene overe...
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ژورنال
عنوان ژورنال: Biotechnology Advances
سال: 2018
ISSN: 0734-9750
DOI: 10.1016/j.biotechadv.2018.10.004